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. 2019 Apr;25(4):465–471. doi: 10.1261/rna.069799.118

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© 2019 Chaker-Margot and Klinge; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — Stage-specific assembly of nucleolar pre-60S particles. (A) SYPRO-Ruby-stained SDS-PAGE gel of preribosomal complexes corresponding to increasing length of large subunit pre-RNA. Ribosome assembly factors are indicated on the right. (B) LC-MS analysis of ribosome assembly factors, based on a triplicate of samples, normalized against Cic1 and classified by recruitment stage. Protein abundance is measured by peak area, and high abundance proteins are shown in orange/red while low abundance and absent proteins are shown in black/gray. (C) LC-MS analysis of the polyadenylate-binding protein (PABP), Smt3, and the H/ACA and box C/D snoRNP proteins.