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Subcellular localization of CYP76B74 in rice protoplasts. Protoplasts without chlorophyll were cotransformed with CYP76B74-GFP and PIN5-mKATE, a marker for the endoplasmic reticulum (ER), and examined using confocal microscopy. A, Green fluorescence indicates the presence of CYP76B74. B, Red fluorescence indicates the presence of ER marker protein. C, Under white light. D, Overlay of these three images indicates that CYP76B74 localizes to the ER.
