Figure 6.

³²P mobility between different leaves in pht1;3 mutants and wild-type plants. The fifth leaf of eight-leaf-old stage plants, which had been treated with Pi-sufficient (HP, 200 μm Pi) or Pi-deficient (NP, 0 μm Pi) nutrient solution for two weeks, was cut at 2 cm from the tip and subsequently exposed to 9 mL of the nutrient solution containing 100 µM Pi labeled with radioactive ³²P. After 30 H, the leaf blades (third to eighth) and shoot basal regions were sampled, then the total ³²P radioactivity of each organ was determined by scintillation counter. Values represent means ± sd of biological replicates (n = 4). Data significantly different from that in corresponding controls are indicated (**P < 0.01, Student’s t test). CPM, counts per minute.