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. 2018 Dec 13;179(2):519–532. doi: 10.1104/pp.18.01228

Figure 4.

Figure 4.

SYP4-dependent accumulation of GFP-VAMP721 at Bgh contact sites. A, Representative three-dimensional (3D) reconstructed images of Arabidopsis leaf epidermal cells expressing GFP-SYP43 and mRFP-VAMP721 at 24 and 48 h post inoculation (hpi) of Bgh. White arrows indicate the extracellular space between the plant and fungal cells. Outlined arrows indicate fungal contact sites. B, Representative 3D reconstructed images of the Arabidopsis leaf epidermal cells expressing GFP-VAMP721 at 24 and 48 hpi of Bgh in the wild-type (wt) and syp42syp43 mutant plants. C, Relative fluorescent intensity at the focal site, outlined arrows in (B), compared with TGNs, white arrowheads in (B), in the wild-type and syp42syp43 cells (n = 23). Asterisks indicate statistical significance based on a Student’s two-sample t test with Bonferroni correction for multiple comparisons (α = 0.05). Bar plots are shown with mean ±sd and raw data points. Scale bars = 10 µm. Lines in the 3D reconstructed images correspond to the x/y/z axes.