Skip to main content
. 2018 Nov 27;179(2):569–587. doi: 10.1104/pp.18.01036

Figure 1.

Figure 1.

Subcellular localization of pSuT-GFP and pSuT hybrid-GFP fusions. Confocal images show transiently transformed Arabidopsis mesophyll protoplasts. From left to right: GFP fluorescence (GFP), chlorophyll autofluorescence (Chl), fluorescence overlay (GFP + Chl), and Nomarski differential interference contrast (DIC). Schematic illustrations of the fusion constructs are shown above the microscopic images. A, Transient expression of pSuT-GFP carrying the native pSuT N terminus with the chloroplast transit peptide (TP) in maximum projection. GFP fluorescence is confined to the plastid envelope membrane (arrow). B, Transient expression of VGT1-GFP with the VGT1 N terminus replaced by the N terminus of pSuT including the chloroplast transit peptide (N-termpSuT-VGT1) in maximum projection. GFP fluorescence is confined to the plastid envelope. C, Transient expression of pSUT-GFP with the pSuT N terminus replaced by the N terminus of VGT1 (N-termVGT1-pSuT) and without the pSuT chloroplast transit peptide in optical sections. Tonoplast targeting of GFP by N-termVGT1-pSuT is supported by gentle lysis of the protoplast and release of the vacuole (Lysis). Arrows indicate the positions of the tonoplast. Bars = 10 µm.