Figure 2.

Growth of yeast W303 cells with 2-dGlc. A and B, Droplet test with yeast cells harboring the empty control vector (−) or the construct expressing a vacuole-targeted pSuT-GFP fusion (Supplemental Fig. S5) lacking its chloroplast transit peptide (ΔTP-pSuT). Cells were grown to an OD₆₀₀ of 1, and serial dilutions were spotted on synthetic complete (SC) agar with 1% (w/v) Glc (A) or 1% (w/v) Glc plus 0.2% (w/v) 2-dGlc (B). C and D, Growth of yeast cells expressing ΔTP-pSuT in liquid SC medium with 2% (w/v) Glc (C) or 2% (w/v) Glc plus 0.2% (w/v) 2-dGlc (D). Data represent means of three independent biological replicates and at least three technical replicates ± sd. E and F, Schematic of the principle of the Glc 2-dGlc uptake assays. The pSuT-dependent sequestration into vacuoles results in the partial detoxification of 2-dGlc. Yellow spheres represent Glc, orange spheres represent 2-dGlc molecules, and green rectangles represent the GFP fusion of ΔTP-pSuT.