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. 2018 Nov 27;179(2):569–587. doi: 10.1104/pp.18.01036

Figure 3.

Figure 3.

Uptake of fluorescent sugar derivatives. 2-NBDG and esculin uptake by yeast W303 cells is shown. The first image in each row (left) shows substrate-dependent fluorescence channels (494–551 nm for 2-NBDG and 465–600 nm for esculin). The second image shows the dark-field image, the third image shows the overlay, and the fourth image (right) shows a schematic representation of the underlying transport processes. Solid arrows point to the luminal side of vacuoles, and dashed arrows point to the cytosol. In the schemes, green spheres represent 2-NDBG, blue spheres represent esculin, green rectangles represent ΔTP-pSuT, and orange rectangles represent SUC2. A, 2-NBDG uptake into cells carrying a control vector. The green 2-NBDG-dependent fluorescence fills a broad zone within the cytosol. B, 2-NBDG uptake into cells carrying an expression vector with ΔTP-pSuT. The green 2-NBDG-dependent fluorescence is concentrated in vacuoles. C, Esculin uptake into cells expressing the plasma membrane Suc transporter SUC2 (PM-SUC2). The cyan esculin-dependent fluorescence fills a broad zone within the cytosol. D, Esculin uptake into cells carrying an expression vector with SUC2 (PM-SUC2) plus an expression vector with ΔTP-pSuT. The cyan esculin-dependent fluorescence is concentrated in vacuoles. Bars = 8.45 µm in A and B and 9.5 µm in C and D.