A, Interaction between RSL Class II protein and RHL1, based on yeast self-activation test. Cells harboring BD–RSL Class II proteins Os07g39940, Os12g39850, Os03g42100, and Os11g41640 were grown on SM media without Trp and uracil. B, Yeast two-hybrid analysis to detect interaction between RSL Class II protein and RHL1. C, Co-IP analysis of interactions between Os07g39940-tagged Myc (Os07g39940–Myc) and RHL1-tagged HA (RHL1–HA), Ehd1 tagged with HA (Ehd1–HA), and Ehd1 tagged with Myc (Ehd–Myc) is shown. C, Ehd1 was used as positive control.