Table 2.
Electrophysiological properties of male and female Drd1a and Drd2 mouse caudate-putamen medium spiny neurons
| Property | Drd1a | Drd2 | Statistics (F, p) |
|---|---|---|---|
| Resting potential, mV | Male: −90.9 ± 1.2a
Female: −87.5 ± 0.7a ,b |
Male: −85.0 ± 1.8b
Female: −89.0 ± 1.2a ,b |
Interaction: F(1,82) = 8.7; p = 0.004
Sex: F(1,82)=0.1; p = 0.75 Subtype: F(1,82)=2.8; p = 0.10 post hoc: Tukey’s |
| Rheobase, nA | Male: 0.16 ± 0.01a
Female: 0.16 ± 0.01a |
Male: 0.10 ± 0.02b
Female: 0.12 ± 0.02a ,b |
Interaction: F(1,82) = 0.4; p = 0.52 Sex: F(1,82)=0.4; p = 0.54 Subtype: F(1,82)=12.3; p = 0.0007 post hoc: Tukey’s |
| AP threshold, mV | Male: −58.3 ± 1.0a,b
Female: −54.6 ± 1.2a |
Male: −58.6 ± 0.7a
,b
Female: −62.6 ± 2.9b |
Interaction: F(1,81)=7.2; p = 0.0087
Sex: F(1,81)=0.01; p = 0.90 Subtype: F(1,81)=8.0; p = 0.0058 post hoc: Tukey’s |
| AP amplitude, mV | Male: 68.8 ± 2.1 Female: 69.5 ± 2.9 |
Male: 69.3 ± 2.8 Female: 79.1 ± 3.1 |
Interaction: F(1,81)=2.3; p = 0.13 Sex: F(1,81)=3.1; p = 0.08 Subtype: F(1,81)=3.0; p = 0.09 |
| AP width at half-peak, ms | Male: 1.63 ± 0.05a
Female: 1.45 ± 0.05a,b |
Male: 1.31 ± 0.06b
Female: 1.43 ± 0.15a,b |
Interaction: F(1,80)=4.7; p = 0.0339
Sex: F(1,80)=0.1; p = 0.7052 Subtype: F(1,80)=5.3; p = 0.0234 post hoc: Tukey’s |
| AHP peak, mV | Male: −8.9 ± 0.4 Female: −11.0 ± 0.7 |
Male: −9.8 ± 0.8 Female: −9.2 ± 0.7 |
Interaction: F(1,80)=3.5; p = 0.0654 Sex: F(1,80)=0.8; p = 0.37 Subtype: F(1,80)=0.2; p = 0.69 |
| AHP time to peak, ms | Male: 32.2 ± 1.3 Female: 26.3 ± 1.6 |
Male: 31.8 ± 3.4 Female: 32.5 ± 3.2 |
Interaction: F(1,81)=2.1; p = 0.15 Sex: F(1,81)=1.4; p = 0.24 Subtype: F(1,81)=1.6; p = 0.21 |
| FI slope, Hz/nA | Male: 192.5 ± 10.4a
Female: 164.0 ± 12.8a |
Male: 278.7 ± 28.9b
Female: 242.0 ± 23.0b |
Interaction: F(1,81)=0.1; p = 0.82 Sex: F(1,81)=3.4; p = 0.0708 Subtype: F(1,81)=21.2; p < 0.0001 post hoc: Tukey’s |
| Linear range input resistance, MΩ | Male: 102.1 ± 5.8a
Female: 93.6 ± 6.6a |
Male: 141.0 ± 19.2b
Female: 109.8 ± 18.3a ,b |
Interaction: F(1,78)=1.1; p = 0.30 Sex: F(1,78)=2.9; p = 0.09 Subtype: F(1,78)=6.3; p = 0.0138 post hoc: Tukey’s |
| Rectified range input resistance, MΩ | Male: 83.4 ± 4.7 Female: 83.9 ± 8.6 |
Male: 112.0 ± 17.7 Female: 83.1 ± 11.7 |
Interaction: F(1,79)=2.0; p = 0.16 Sex: F(1,79)=1.9; p = 0.17 Subtype: F(1,79)=1.8; p = 0.18 |
| Inward rectification, % | Male: 82.3 ± 1.3 Female: 81.0 ± 1.8 |
Male: 80.8 ± 4.3 Female: 79.2 ± 3.3 |
Interaction: F(1,79)=0.0; p = 0.93 Sex: F(1,79)=0.3; p = 0.57 Subtype: F(1,79)=0.4; p = 0.52 |
| Time constant of the membrane, ms | Male: 10.3 ± 0.8 Female: 19.6 ± 1.2 |
Male: 14.6 ± 3.2 Female: 9.9 ± 1.9 |
Interaction: F(1,82)=2.9; p = 0.09 Sex: F(1,82)=1.2; p = 0.28 Subtype: F(1,82)=1.6; p = 0.20 |
| Capacitance, pF | Male: 109.6 ± 10.0 Female: 99.0 ± 7.0 |
Male: 94.4 ± 11.2 Female: 91.2 ± 8.5 |
Interaction: F(1,78)=0.1; p = 0.74 Sex: F(1,78)=0.4; p = 0.55 Subtype: F(1,78)=1.0; p = 0.31 |
| mEPSC frequency, Hz | Male: 1.9 ± 0.2 Female: 1.9 ± 0.2 |
Male: 2.0 ± 0.5 Female: 1.4 ± 0.4 |
Interaction: F(1,47)=0.9; p = 0.33 Sex: F(1,47)=1.2; p = 0.29 Subtype: F(1,47)=0.3; p = 0.58 |
| mEPSC amplitude, pA | Male: 15.9 ± 0.4a
Female: 15.2 ± 0.6a |
Male: 17.7 ± 1.1a
,b
Female: 20.7 ± 1.5b |
Interaction: F(1,47)=5.2; p = 0.0270
Sex: F(1,47)=2.1; p = 0.1498 Subtype: F(1,47)=20.4; p < 0.0001 post hoc: Tukey’s |
| mEPSC decay, ms | Male: 1.9 ± 0.2 Female: 1.9 ± 0.2 |
Male: 2.0 ± 0.5 Female: 1.4 ± 0.4 |
Interaction: F(1,47)=2.6; p = 0.11 Sex: F(1,47)=0.1; p = 0.76 Subtype: F(1,47)=0.0; p = 0.95 |
Values are mean ± SEM. Bold font indicates statistical significance. Different superscript letters denote significant differences detected by a Tukey’s post hoc test. AP, action potential; AHP, afterhyperpolarization; FI, frequency of evoked spikes to injected depolarization current.