Table 5.

Number of Lhx1+5/Evx2/Maf/Esrrg quadruple-positive neurons at different developmental stages in the mouse

Stage	r5-cMVST lateral	Medial extension	N preparations	Sections assessed/preparation
E9.5	0	0	3	Every 4th
E11.5	8	2	1	Every 4th
E13.5a	85 ± 25	25 ± 21	3	Every 6th
E15.5b	98 ± 21	25 ± 9	3	Every 6th

Counts of quadruple positive cells ± SD in different areas of the mouse CNS.

^aCounts represent entire CNS. Only the hindbrain was assessed at other stages.

^bPreparations at E15.5 were not coimmunostained for Lhx1+5 because they included retrograde labeling of the cMVST; here, counts represent Evx2/Maf/Esrrg immunostained neurons in and around the cMVST domain only (other brainstem areas were not assessed; Fig. 9).