Video 3. Dynamic recruitment of the stress granule marker GFP-TIA1 into light-induced Opto-G3BP1 granules.

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DOI: 10.7554/eLife.39578.009

U2OS cells transiently transfected with Opto-G3BP1 and the stress granule marker GFP-TIA1 were stimulated with a blue-light laser (power density ~2.5 MW/cm²) for 5 msec. Cells were sequentially imaged by 561 nm and 488 nm channels; we note that the 488 nm channel used for imaging (power density 2.2 W/cm²) also activates Opto-G3BP1.