Video 5. Blue light activation induces the assembly of OptoGranules in iPSC-derived neurons expressing Opto-G3BP1.
iPSC-derived neurons expressing Opto-G3BP1 (mRuby) were intermittently exposed to a 488 nm blue-light laser (95% laser power, power density 6.5 W/cm2) followed by image acquisition with a 561 nm channel. See Video 4 for corresponding Opto-Control condition.