Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Human) | U-2 OS | ATCC | HTB-96; RRID:CVCL_0042 | |
| Cell line (Human) | Lenti-X 293T(293LE) | Clontech | 632180; RRID: CVCL_4401 | |
| Cell line (Human) | iPSCs | Building a Kidney | BJFF6; RRID: CVCL_VU02 | |
| Recombinant DNA reagent | pCRY2PHR-mCherryN1 | Addgene | 26866; RRID:Addgene_26866 | |
| Recombinant DNA reagent | pCMV-CRY2-mCherry | Addgene | 58368; RRID:Addgene_58368 |
|
| Recombinant DNA reagent | phND2-N174 | Addgene | 31822; RRID:Addgene_31822 |
|
| Recombinant DNA reagent | pKanCMV-mClover3-mRuby3 | Addgene | 74252; RRID:Addgene_74252 |
|
| Recombinant DNA reagent | pTight-hND2-N106 | Addgene | 31875; RRID:Addgene_31875 | |
| Recombinant DNA reagent | psPAX2 | Addgene | 12260; RRID:Addgene_12260 |
|
| Recombinant DNA reagent | CRY2olig-mCherry | Addgene | 60032; RRID:Addgene_60032 | |
| Recombinant DNA reagent | pMD2.G | Addgene | 12259; RRID:Addgene_12259 |
|
| Recombinant DNA reagent | linear hygromycin marker | Clontech | 631625; RRID:Addgene_60032 | |
| Antibody | goat polyclonal anti-β-actin |
Santa Cruz Biotechnology |
sc-1616; RRID: AB_630836 | (1:1000) |
| Antibody | mouse monoclonal anti-eIF2α | Santa Cruz Biotechnology |
sc-133132; RRID: AB_1562699 | (1:1000) |
| Antibody | rabbit polyclonal anti-β-actin | Cell Signaling | 3597S; RRID: AB_390740 | (1:1000) |
| Antibody | rabbit polyclonal anti-mCherry | Abcam | 167453; RRID: AB_2571870 | (1:1000) |
| Antibody | mouse monoclonal anti-G3BP1 | BD Biosciences | 611126; RRID: AB_398437 | (1:1000) |
| Antibody | rabbit polyclonal anti-PABP | Abcam | ab21060; RRID: AB_777008 | (1:400) |
| Antibody | rabbit polyclonal anti-eIF4G | Santa Cruz Biotechnology |
sc-11373; RRID: AB_2095750 |
(1:400) |
| Antibody | rabbit polyclonal anti-TDP-43 | Proteintech | 12892–1-AP; RRID: AB_2200505 | (1:400) |
| Antibody | mouse monoclonal anti-phospho-TDP-43 (M01) |
Cosmo Bio CO | TIP-PTD-MO1; RRID: AB_1961900 | (1:1000) |
| Antibody | rabbit polyclonal anti-phospho-TDP-43 (P01) | Cosmo Bio CO | TIP-PTD-PO1; RRID: AB_1961899 | (1:400) |
| Antibody | mouse monoclonal anti-VCP | BD Biosciences | 612183; RRID: AB_399554 | (1:100) |
| Antibody | rabbit polyclonal anti-amyloid-oligomer A11 | Thermo Fisher Scientific |
AHB0052; RRID: AB_2536236 | (1:100) |
| Antibody | rabbit polyclonal anti-Ubiquitin | Dako | Z0458; RRID: AB_2315524 |
(1:100) |
| Antibody | mouse monoclonal anti-p62 | Abcam | ab56416; RRID: AB_945626 |
(1:400) |
| Antibody | mouse monoclonal anti-MAP2 | Sigma | M9942; RRID: AB_477256 |
(1:400) |
| Antibody | goat polyclonal anti-TIA1 |
Santa Cruz Biotechnology | sc-1751; RRID: AB_2201433 | (1:400) |
| Antibody | mouse monoclonal anti-TIAR | BD Biosciences | 610352; RRID: AB_397742 | (1:400) |
| Antibody | rabbit polyclonal anti-ataxin 2 | Proteintech | 21776–1-AP; RRID: AB_10858483 | (1:400) |
| Antibody | goat polyclonal anti-eIF3η |
Santa Cruz Biotechnology |
sc-16377; RRID: AB_671941 |
(1:400) |
| Antibody | rabbit polyclonal anti- GLE1 |
Abcam | ab96007; RRID: AB_10678755 | (1:400) |
| Antibody | rabbit polyclonal anti- FUS |
Bethyl Laboratories |
A300-302A; RRID: AB_309445 | (1:400) |
| Antibody | rabbit polyclonal anti- OPTN |
Proteintech | 10837–1-AP; RRID: AB_2156665 | (1:400) |
| Commercial assay or kit |
FuGENE 6 | Promega | E2691 | |
| Commercial assay or kit | NEBuilder HiFi DNA Assembly Master Mix kit |
NEB | E2621 | |
| Commercial assay or kit | Q5 site-directed mutagenesis |
NEB | E0054 | |
| Commercial assay or kit | RNA 3′ End Biotinylation Kit |
Pierce | 20160 | |
| Commercial assay or kit | CellTiter-Glo 2.0 assay kit | Promega | G9242 | |
| Chemical compound, drug | ISRIB | Sigma | SML0843 | 200 nM |
| Chemical compound, drug | cycloheximide | Sigma | C4859 | 100 µg/ml |
| Chemical compound, drug | sodium arsenite | Sigma | 35000–1 L-R | 0.5 mM |
| Chemical compound, drug | hygromycin B | Thermo Fisher Scientific |
10687010 | 200 μg/ml |
| Chemical compound, drug | doxycycline hyclate | Sigma-Aldrich | D9891 | 1 µg/ml |
| Chemical compound, drug | puromycin | Thermo Fisher Scientific |
A1113803 | 1 µg/ml |
| Software, algorithm | ImageJ | NIH | https://imagej.nih.gov/ij/, RRID:SCR_003073 | |
| Software, algorithm | GraphPad Prism | GraphPad Software Inc |
http://www.graphpad.com/
scientific-software/prism/ RRID:SCR_002798 |
|
| Software, algorithm | SlideBook 6 | Intelligent Imaging Innovations |
https://www.intelligent-imaging.com/slidebook.php
RRID:SCR_014300 |
|
| Software, algorithm | Image Studio | LI-COR |
https://www.licor.com/bio/products/software/image_studio_lite/?utm_source=BIO+Blog&utm_medium=28Aug13post&utm_content=ISLite1&utm_campaign=ISLite, RRID: SCR_014211
RRID:SCR_015795 |
|
| Software, algorithm | LAS X Software | Leica |
https://www.leica-microsystems.com/products/confocal-microscopes/p/leica-tcs-sp8/
RRID:SCR_013673 |