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ELISA curves for 18 positive clones. The sensitivity of the assay varied in the range of 6 to 50 ng/mL. Conditions: pAb anti-Cif 0.3 μg/well; serial dilution of Cif in PBS; VHH 50 ng/well in PBS; anti-HA mAb dilution 1:3000 in PBST.