TABLE 2.
ATP content in SAR11 strains in the presence of DMA, MMA, or pyruvatea
| SAR11 strain | Ratio of ATP (SD) with: |
|||||
|---|---|---|---|---|---|---|
| Pyruvate |
DMA |
MMA |
||||
| 10 μM | 30 μM | 10 μM | 30 μM | 10 μM | 30 μM | |
| HTCC7211 | 2.77b ,c (0.34) | 3.49b ,c (0.36) | 2.20b (0.38) | 2.08b (0.40) | 1.74b (0.20) | 0.83 (0.08) |
| HTCC1062 | 2.76b ,c (0.05) | 3.36b ,c (0.09) | 1.46b (0.03) | 1.49b (0.04) | 1.00 (0.02) | 1.36 (0.05) |
a
Data are expressed as the ratio between treatments with arsenic compounds and energy-starved controls. Cells were harvested in late exponential phase, washed once with ASW, resuspended in 2 ml ASW, and starved for 16 h. Subsamples were incubated with substrates for 28 h and then assayed for ATP content. Numbers in parentheses are SD determined by propagation of error.
b
P value significance (pairwise t test) was <0.05, compared to the value for the negative control for the given strain.
c
P value significance (pairwise t test) was <0.05, compared to values with DMA or MMA for the given strain.