FIG 4.

CarolAnn genes 43 and 44 confer defense against phage infection. (A) The organization of CarolAnn genes 41 to 46 is shown at the top, with arrows indicating the positions of putative promoters and the direction of transcription. Genes are represented as boxes, with the gene names within the box; the Phamily designation and the number of Pham members (in parentheses) are shown below each box. The segments of CarolAnn DNA present in plasmids pMM15, pMM16, pMM18, and pMM59 are shown as black lines. Plasmids pMM55 and pMM56 contain the same CarolAnn genes as plasmids pMM16 and pMM59, respectively, but the genes are inserted into Tet-ON vector pCCK39 (Table S2). (B) Lysates of phages Bialota, BatStarr, Nymphadora, Yeezy, CarolAnn, KatherineG, OneUp, and Kita were serially diluted 10-fold and plated onto lawns of G. terrae strains. Plasmid pMM16 confers defense against all of the phages except CarolAnn. (C) Dilutions of the same phages were plated onto lawns of G. terrae 3612 on solid medium either lacking (Uninduced) or containing (Induced) ATc inducer.