FIG 1.

AP-2 is present at sites of endocytosis in Candida albicans. (A) Representative images of yeast and 90-min hyphae showing Apl1-GFP puncta. (B) Analysis of times Apl1-GFP puncta are present at cell surface, in strain with single tag; n = 30 puncta measured for each condition. Error bars show means and SDs. (C) Representative images of coexpressed Sla1-GFP and Apl1-RFP. Arrowheads show examples of puncta where signals colocalize. (D) Intensities of Sla1-GFP and Apl1-RFP in a representative surface punctum over time. Images below graph show this punctum every 8 s, corresponding to time axis on graph. (E) More than one hundred peripheral puncta were counted in both yeast and hyphal cells, and whether they contained Apl1-RFP signal, Sla1-GFP signal, or both signals colocalized was recorded. Graph shows percentages of all puncta counted.