FIG 10.

Distinct requirements for ergosterol and mannose polarization. (A) Hyphae were induced for 60 min and then stained with filipin, which binds ergosterol; the percentages of cells which showed a bright ergosterol-rich domain at the hyphal tip were recorded for each strain (n > 200 cells/strain from 3 independent experiments). Statistics are from two-way ANOVAs with Tukey’s post hoc tests. Error bars show SDs. (B) Representative images of filipin-stained hyphae from each strain. (C) Representative images in which stationary-phase yeast were stained with concanavalin-A-Alexa 594 and then induced to form hyphae for 90 min and stained with ConA-Alexa 488. Scale bars, 5 μm. ns, P = 0.1234; ****, P < 0.0001.