FIG 2.

AP-2 disruption does not significantly affect endocytic site organization or fluid-phase uptake. (A) Growth curves of WT and apm4 deletion cells over 8 h; 3 biological and 3 technical replicates per strain in 3 separate experiments. (B) Fluid-phase endocytosis assay; cells incubated with Lucifer yellow dye (LY) for 60 min and uptake quantified by fluorimetry. Graph shows uptake normalized to total cell protein. Images are representative of LY uptake in each strain; 3 biological and 3 technical replicates per strain in 3 independent experiments. (C) Representative images of yeast cells stained with rhodamine phalloidin showing actin patches and cables; quantification of number of actin patches in mother and bud of each cell (n = 20 cells per strain; 1 representative experiment). (D) Images of hyphal cells stained with rhodamine phalloidin. Quantification of fluorescent signal at hyphal tip divided by that at the “base” of the hypha (closest to mother cell); n = 20 cells per strain. Statistics in panels B to D are unpaired t tests of data. All error bars represent SD. ns, P = 0.1234.