FIG 3.

AP-2 disruption affects cell morphology. (A) Representative images of WT, heterozygous, and homozygous apm4 deletion strains, grown as hyphae for 2 h and then stained with calcofluor white. Scale bars, 5 μm. Measurements of hyphal length (n > 100 cells/strain from 2 independent experiments) (B) and hyphal diameter at the “neck” (n > 75 cells/strain; 1 experiment) (C) in WT and apm4 deletion strains. (D) Metabolic activity as measured by XTT assay of cells grown as biofilms on plastic, after washing. (E) Representative images of biofilms after washing, taken on a light microscope with a 20× objective. All statistics are from one-way ANOVAs with Tukey’s post hoc tests. (B, C) Central lines at medians; whiskers at minimum and maximum values. (D) Error bars show SDs. ns, P = 0.1234; ****, P < 0.0001.