FIG 4.

AP-2 disruption affects ability of cells to undergo polarized growth. (A) Quantification and representative images of hyphal cells stained with filipin (n > 300 cells/strain; 4 independent experiments). Statistics are from a two-way ANOVA with Sidak’s post hoc test. Error bars show SDs. (B) Stationary-phase yeast were stained with concanavalin-A-Alexa 594 (red) and then induced to form hyphae for 90 min and stained with ConA-Alexa 488 (green). Representative images shown. Scale bars, 5 μm. ****, P < 0.0001.