FIG 7.

AP-2 colocalizes with Chs3 and its depletion impairs Chs3 uptake. (A) Representative images of yeast cells in which Chs3 was tagged with GFP and Apl1 with RFP. White arrowheads indicate examples of the two signals colocalizing at peripheral puncta. (B) Intensity profiles of lines drawn across representative puncta from 10 cells. Values shown are normalized to background fluorescence and expressed as a fraction of the peak intensity for each line. Error bars show SDs. (C) Representative images of Chs3-GFP fluorescence in APM4 WT and apm4-deleted cells grown as yeast or 90-min hyphae. Scale bars, 5 μm.