FIG 8.

Deletion of one copy of chs3 rescues some of the apm4 mutant phenotypes. (A) Quantification of fluorescence intensity of CFW-stained yeast cells; the mean gray value, minus background, was measured with FIJI for each cell. Widths of yeast cells (B) and lengths (C) and widths (D) of hyphae measured with FIJI after growth for 90 min (n = 60 cells per strain for panels A to D, from 2 independent experiments). (E) Representative images of yeast and hyphae stained with CFW. All statistics are from one-way ANOVAs with Tukey’s post hoc tests. Error bars show SDs. Scale bars, 5 μm. ns, P = 0.1234; *, P = 0.0332; **, P = 0.0021; ****, P < 0.0001.