FIG 9.

Apm4 binds Chs3 through its YXXΦ binding domain and truncation of this domain leads to morphological defects. Quantification of hyphal lengths (A), hyphal widths (B), and yeast cell widths (C) in apm4 cargo-binding mutant compared to those from heterozygous and homozygous apm4 deletion strains. (D) Representative images of yeast and hyphae stained with CFW. (E) Quantification of CFW staining intensity in these strains (mean gray value minus background for each cell). (F) Chs3 tagged with GFP in each strain. (G) Signals quantified by line profiles drawn from insides to outsides of 20 cells/strain; (A to C and E) n = 80 cells/strain from 3 independent experiments. (E) Values are normalized to the mean Δapm4 measurement. Statistics are from one-way ANOVAs with Tukey’s post hoc tests. Error bars show SDs. Scale bars, 5 μm. ns, P = 0.1234; **, P = 0.0021; ****, P < 0.0001.