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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Autoimmun. 2019 Jan 3;98:33–43. doi: 10.1016/j.jaut.2018.11.004

Figure 3. NTN is successfully induced in myeloid RelA KO mice.

Figure 3.

To ensure that knocking out RelA did not interfere with disease induction, we measured antibodies generated in response to the initial rabbit IgG immunization in WT and KO mice in terminal serum(A). Isotype specific mouse anti-rabbit IgG antibody titers (IgG1, IgG2a, IgG2c, and IgG3 levels) are shown in (B-E), with no difference seen in any of the isotypes. Anti-GBM antibody titers were measured following passive transfer of the nephrotoxic serum to ensure that both mouse genotypes received similar doses and had similar clearance (F). Shown are the results from 3 separate cohorts (HC, n=4; RelA KO, n = 19, RelA WT, n = 17). Data for mouse anti-rabbit IgG and anti-GBM titers were analyzed using an ordinary one-way ANOVA, and multiple comparisons were determined using Tukey’s test. To assess the various IgG isotypes, which were normally distributed, a student’s T-test was used.