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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Autoimmun. 2019 Jan 3;98:33–43. doi: 10.1016/j.jaut.2018.11.004

Figure 5. Cytometric analysis of renal immune cells and splenic cell populations.

Figure 5.

(A) Myeloid-specific RelA KO mice had similar populations of kidney myeloid cells as WT mice, when assessed by flow cytometry at baseline (pre-disease induction). (B) A significant reduction of M1 inflammatory macrophages was found in the kidneys of myeloid RelA KO mice compared to WT mice. In contrast, increased M2 tissue resident macrophages were found in the former strain. This trend was not only true for total cell number, but also when looking at proportions of immune cells in the kidneys (% CD45+), as shown in C. There was no difference in T and B cell renal populations (D). In the spleen, there were increased numbers of M1 monocytes in myeloid RelA KO mice, indicating that the splenic reservoir was not being depleted in this strain (E). There was no difference in T and B cells in the spleen (F). Shown are results from one cohort (RelA KO, n = 5; RelA WT, n = 5). %SC, % single cells. Significance was assessed using a student’s T-Test.