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. Author manuscript; available in PMC: 2020 Mar 1.

Published in final edited form as: J Autoimmun. 2019 Jan 3;98:33–43. doi: 10.1016/j.jaut.2018.11.004

Figure 6. — (A) We measured protein cytokine levels in whole kidney protein extracts using Legendplex. There was decreased expression of IL-1a, IFNg, and IL-6 in KO mice, whereas IL-10 was increased. This pattern of cytokine expression is consistent with the larger population of resident macrophages as opposed to inflammatory macrophages. Outliers were assessed using Grubb’s test. Data was analyzed using a student’s T-test (B) Macrophages were isolated from KO and WT mice, cultured, and stimulated with LPS. Legendplex was performed on the supernatants. Cells were isolated from 3 mice per group, and plated in duplicate for stimulation. Data was analyzed using a two-way ANOVA and Tukey’s test for multiple comparisons. (C) RT-PCR was performed on RNA isolated from whole kidneys to assess the expression of IFN response genes. Data was analyzed using an ordinary one-way ANOVA and Tukey’s test for multiple comparisons. (For the kidney protein levels and RT-PCR, shown are the results from 2 cohorts; HC, n = 4; RelA KO, n = 10, RelA WT, n = 10)

Figure 6. — (A) We measured protein cytokine levels in whole kidney protein extracts using Legendplex. There was decreased expression of IL-1a, IFNg, and IL-6 in KO mice, whereas IL-10 was increased. This pattern of cytokine expression is consistent with the larger population of resident macrophages as opposed to inflammatory macrophages. Outliers were assessed using Grubb’s test. Data was analyzed using a student’s T-test (B) Macrophages were isolated from KO and WT mice, cultured, and stimulated with LPS. Legendplex was performed on the supernatants. Cells were isolated from 3 mice per group, and plated in duplicate for stimulation. Data was analyzed using a two-way ANOVA and Tukey’s test for multiple comparisons. (C) RT-PCR was performed on RNA isolated from whole kidneys to assess the expression of IFN response genes. Data was analyzed using an ordinary one-way ANOVA and Tukey’s test for multiple comparisons. (For the kidney protein levels and RT-PCR, shown are the results from 2 cohorts; HC, n = 4; RelA KO, n = 10, RelA WT, n = 10)