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. Author manuscript; available in PMC: 2020 Mar 1.
Published in final edited form as: J Autoimmun. 2019 Jan 3;98:33–43. doi: 10.1016/j.jaut.2018.11.004

Table 1.

Antibodies used for staining.

IF STAINING
primary dilution secondary dilution
Stain 1 goat anti- mouse C3 1:100 donkey anti-goat AF594 1:100
rabbit anti-mouse IBA-1 1:250 donkey anti-rabbit AF488 1:250
donkey anti-mouse IgG 1:500 donkey anti-mouse IgG 1:500
Stain 2 rat anti- mouse B220 1:100 donkey anti-rat AF594 1:100
rabbit anti-mouse CD4 1:100 donkey anti-rabbit AF488 1:100
Stain 3 rat anti-mouse Ly6G 1:100 donkey anti-rat AF594 1:100
FLOW CYTOMETRY
Stain 1 Ly6C (PE) 1:1000
F480 (PerCP) 1:100
CD45 (FITC) 1:100
CD11b (AF700) 1:100
CD11c (Pacific Blue) 1:100
Stain 2 CD8 (FITC) 1:101
B220 (PE) 1:102
CD45 (PerCP) 1:103
CD4(APC) 1:104