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. 2019 Mar 19;26(12):3369–3379.e5. doi: 10.1016/j.celrep.2019.02.074
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Mouse anti-human CD3 (OKT3) eBioscience Cat#16-0037-81
Mouse anti-human CD3 (UCHT1) eBioscience Cat#16-0038-81
Chemicals, Peptides, and Recombinant Proteins
HLA-A2 MHC Aleksic et al., 2010 N/A
NY-ESO-1156−157 9V Generon UK N/A
NY-ESO-1156−157 4D Generon UK N/A
ICAM1, hIgG1-Fc.His Tag Thermo Fisher Cat#10346H03H50
DOPC Avanti Polar Lipids Cat#850375
Ni2+-NTA-DGS Avanti Polar Lipids Cat#790404
Cap biotin PE Avanti Polar Lipids Cat#870273
NaCl Sigma-Aldrich Cat#S9888
KCl Sigma-Aldrich Cat#P9541
Glucose Sigma-Aldrich Cat#G8270
CaCl2 Sigma-Aldrich Cat#449709
MgCl2 Sigma-Aldrich Cat#M8266
Casein Sigma-Aldrich Cat#C7078
NiCl2 Sigma-Aldrich Cat#339350
Streptavidin Sigma-Aldrich Cat#85878
Jasplakinolide Sigma-Aldrich Cat#J4580
Y27632 Sigma-Aldrich Cat#Y0503
RPMI-1640 Sigma-Aldrich Cat#R8758
FBS Sigma-Aldrich Cat#F9665
Human Serum Albumin (HSA) Sigma-Aldrich Cat#SRP6182
Penicillin-streptomycin Sigma-Aldrich Cat#P4333
L-glutamine Lonza Cat#17-605E
HEPES Sigma-Aldrich Cat#H0887
Sodium Pyruvate Lonza Cat#13-115E
Poly-L-Lysine Sigma-Aldrich Cat#P8920
EZ-Link Sulfo-NHS-LC-Biotin Thermo Fisher Cat#21335
Sulfo-SANPAH Thermo Fisher Cat#22589
Streptavidin-acrylamide Thermo Fisher Cat#S21379
Biotinylated Bovine Serum Albumin Sigma-Aldrich Cat#A8549
Bovine Serum Albumin Sigma-Aldrich Cat#A2153
CellMask Deep Red Thermo Fisher Cat#C10046
Cholestorol-PEG-KK114 Honigmann et al., 2014 N/A
40nm red (594/620) fluorescent beads Invitrogen Cat#F8793
SNAP-cell-505 NEB Cat#S9103S
Glutaraldehyde Sigma-Aldrich Cat#340855
APTMS Sigma-Aldrich Cat#281778
APS Sigma-Aldrich Cat#A3678
TEMED Sigma-Aldrich Cat#T9281
N,N’-Methylenebisacrylamide solution (2%) Sigma-Aldrich Cat#M1533
Acrylamide solution (40%) Sigma-Aldrich Cat#A4058
Experimental Models: Cell Lines
Jurkat 1G4-TCR T cell Lee et al., 2017 N/A
Recombinant DNA
Lifeact-mCitrine Addgene 54733
Software and Algorithms
ABAQUS 6.124-3 ABAQUS, Inc. N/A
MATLAB 2018a MathWorks, Inc. N/A
Trackpy N/A https://soft-matter.github.io/trackpy/v0.3.2/)
PicoQuant SymPhoTime PicoQuant N/A