Table 1.

A comparison of media described for the maintenance of isolated nephron progenitors. Adapted from Li et al [30].

		NPSR		NPEM		CDBLY
		Li et al. 2016		Brown et al. 2015		Tanigawa et al. 2016
Culture Period		Until now, more than 17 months and more than 110 passages		Up to 10 passages were shown		Up to around 19 days
Culture Condition	Format	3D		2D (Matrigel coating)		2D (iMatrix coating)
	Basal Medium	DMEM/F12		APEL		DMEM/F12
	Specific Factors	Factor	Final Concentration	Factor	Final Concentration	Factor	Final Concentration
		BMP7	50ng/ml	BMP7	30ng/ml	TGF-α	10ng/ml
		FGF2	200ng/ml	FGF9	200ng/ml	FGF2/9	50ng/ml
		Heparin	1ug/ml	Heparin	1ug/ml	LIF	5ng/ml
		Y27632	10uM	Y27632	10uM	Y27632	10uM
		CHIR99021	1uM	CHIR99021	1.25uM	CHIR99021	1uM
		LIF	10ng/ml	LDN193189	125nM	BMP7	5ng/ml
				BMP4	30ng/ml	DAPT	2.5uM
				IGF1	20ng/ml
				IGF2	2ng/ml