Figure 6.

siRNA Internalization and Activity in FACS-Isolated Lung Epithelial and Endothelial Cells

(A) Expression of cell-specific markers for endothelial cells (Kdr, Icam2, Pecam1), club cells (Cyp2f2, Scg1a1), type I (Pdpn), and type II (Sftpa1) epithelial cells in dissociated lung tissue before (total lung) and after FACS-purification of CD45^negCD326⁺CD31^neg (epithelial) and CD45^negCD31⁺ (endothelial) cell subsets. (B) Ctnnb1 mRNA levels were determined in dissociated lung tissue and FACS-isolated subsets 72 h post-dosing of 15 mg/kg siRNA. (C) siRNA content was determined by stem-loop PCR. Lines indicate means ± SE. **p < 0.005 versus PBS-treated cell subset, ###p < 0.0005 versus total lung cells.