Figure 7.

Administration of siRNA Targeting OX40L Impairs Th2 Response in the OVA-Induced Lung Inflammation Model

(A) Experimental timeline. (B) Tnfsf4 (OX40L) mRNA levels were determined in total lung RNA from mice dosed with siRNA targeting Tnfsf4 or siRNA control. (C) Th2 cytokine mRNA levels in BAL fluid were determined by RT-PCR. Lines indicate means ± SE. (D) Th2 cytokine protein levels in BAL fluid. (E) Serum OVA-specific IgE were determined by ELISA. Data was analyzed using one-way ANOVA with post-hoc Dunnett’s test. Data are means + SE. *p < 0.05, **p < 0.005 versus challenged, PBS-dosed control group; #p < 0.05, ##p < 0.005, n.s., not significant.