Distribution of Donor mtDNA in the Blastomeres of Pre-implantation Embryos Shared the Same Tendency as that in Adult Tissues
(A) Schematic model of embryos and dissected blastomeres of embryos at 2-cell stage. Heteroplasmy distribution of donor mtDNA in blastomeres of embryos at 2-cell stage from heteroplasmic mice.
(B) Schematic model of embryos and dissected blastomeres of embryos at 4-cell stage. Heteroplasmy distribution of donor mtDNA in blastomeres of embryos at 4-cell stage from heteroplasmic mice.
(C) Schematic model of embryos and dissected blastomeres of embryos at 8-cell stage. Heteroplasmy distribution of donor mtDNA in blastomeres of embryos at 8-cell stage from heteroplasmic mice.
(D) Negative correlation between donor mtDNA heteroplasmy (green) and V′(h) (red) values in blastomeres of embryos at 2, 4, and 8-cell stage (r = −0.45, p < 0.05 for 2-cell stage; r = −0.58, p < 0.0001 for 4-cell stage; r = −0.48, p < 0.005 for 8-cell stage. Spearman correlation test).
Error bars indicate SD, with the mean value. See also Tables S4–S6.