Figure 3.

No selective replication of donor mtDNA took place during the progressive cleavage across developmental stages

(A and B) (A) Spread of donor mtDNA heteroplasmy from embryonic blastomere to adult tissue expressed by heteroplasmy range (maximum or minimum heteroplasmy value minus the mean median of heteroplasmy value). The mean median is defined as half of the sum of maximum and minimum values. Light bar = maximum − median = maximum − $\frac{\text{maximum }+\text{ minimum}}{2}$ = $\frac{\text{maximum }-\text{ minimum}}{2}$; dark bar = $\text{minimum}-\text{median}=\text{minimum}-\frac{\text{maximum }+\text{ minimum}}{2}=-\frac{\text{maximum }-\text{ minimum}}{2}$ (B) V′(h) values of embryonic blastomeres and adult tissue.

(C) Comparison of the spread ranges among embryos at 2-, 4-, and 8-cell stage and adults. Data are represented as mean ± SD.

(D) Comparison of V′(h) values among embryos at 2-, 4-, and 8-cell stage and adults. Different letters indicate p values < 0.05 (Mann-Whitney test); error bars indicate SD. See also Tables S3–S6.

(E) Comparison of mean heteroplasmy values of embryos at 2-, 4-, and 8-cell stage and adult tissues (p > 0.05, Mann-Whitney test). Data are represented as scatterplot with mean ± SD.

(F) Frequency histogram of the donor mtDNA heteroplasmy of embryos at 2-, 4-, and 8-cell stage and adult tissues.

(G) Cumulative probability distribution functions for the heteroplasmy of embryos at 2-, 4-, and 8-cell stage and adult tissues. (p > 0.05, Kolmogorov-Smirnov test).

See also Tables S3–S6.