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. 2019 Mar 14;10:178. doi: 10.3389/fgene.2019.00178

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Copyright © 2019 Jones, Lingampally, Dilai, Shrestha, Stripp, Helmbacher, Chen, Chao and Bellusci.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Model to inhibit FGF10 in vivo and in vitro demonstrates Etv4 and Etv5 are downstream of FGF10/FGFR2b signaling (A) In vivo model to inhibit FGF10/FGFR2b signaling: pregnant females carrying experimental (Rosa26^rtTA/rtTA; Tg^Tet(o)sR2b/+) and littermate control (Rosa26^rtTA/rtTA; Tg^+/+) embryos were injected with doxycycline. Embryonic lungs were isolated 9 h later. Scale bar: 500 μm. (B) Corresponding RT-qPCR analysis showing Etv4 and Etv5 expression in experimental vs. control lungs. (C) In vitro model to inhibit FGF10/FGFR2b signaling: E12.5 lung explants were cultured for 24 h with (experimental) or without (control) 5 μg/ml recombinant (soluble) FGFR2b. Scale bar: 500 μm. (D) Corresponding RT-qPCR analysis showing Etv4 and Etv5 expression in experimental vs. control lungs. (Data are presented as mean ± SEM; ^*p < 0.05).