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. 2019 Mar 14;10:477. doi: 10.3389/fmicb.2019.00477

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Copyright © 2019 Gao, Li, Wan, Hu, Liu, Yang, Gong, Zeng, Wei, Yang, Zeng, He, Huang and Cao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protective effect of HM0539 on intestinal barrier function. (A–C) Caco-2 monolayers were pretreated with different concentrations of HM0539 (0–100 ng/ml), the soluble mucin (A) and mucin-covered Caco-2 surface (B) were detected by PAS assay. Western blot (C, upper panel) and densitometric analysis (C, lower panel) of MUC2 expression. (D–F) Caco-2 monolayers were pretreated with HM0539 or Benzyl-α-GalNAc (mucin inhibitor), then stimulated with LPS (1 μg/ml) or TNF-α (10 ng/ml) for 6 h. (D) Western blot analysis of MUC2, ZO-1 and occludin, β-actin was used as loading control. (E) Immunofluorescent staining of ZO-1. (F) TEER were detected to evaluate the intestinal barrier integrity. Data are given as means ± SEM; ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001; NS, no statistical significance.