Fig. 5.

Tau expression reduces pS6 in immortalized human cells. a Representative immunoblot showing the effects of tau expression in tetracycline-inducible iHEK-Tau cells. Tau was expressed for 24 or 96 h (ON) and either harvested immediately or following 24 h tetracycline wash-out (OFF). Quantification of the ratio of PHF1 to total tau (H150 antibody) levels (b) or pS6 to total S6 levels (c). Data analyzed by two-way ANOVA with Tukey’s multiple comparisons test. **p < 0.01, ***p < 0.001, ****p < 0.0001