Figure 4.

AMH gene expression and protein in adult ovary of pAmh WT, pAmh GATAmut, and pAmh del40 mice. (A) Amh expression was assessed by quantitative RT-PCR at PND120. Data are reported as the value relative to the age-matched pAmh WT mouse line (n = 8 to 20). The open circle is the lone outlier. No statistically significant differences were found between the pAmh WT and pAmh GATAmut or pAmh del40 mice (P < 0.05). (B) Immunohistochemistry was performed on serial paraffin ovary sections obtained from each mouse genotype at PND120 using an antibody specific for AMH (αAMH). For each genotype analyzed, the images shown represent results from tissue sections that were processed under identical conditions and on the same day. Original magnification, ×50. f, follicle.