Skip to main content
. 2019 Jan 17;10(2):43. doi: 10.1038/s41419-018-1282-6

Fig. 1. Twist1 promotes the transcription of the thymidine phosphorylase (TP) gene by binding to the conserved motifs in the promoter region.

Fig. 1

a Heat map of Twist1 co-expression genes downloaded from The Cancer Genome Atlas (TCGA) database. b Venn analysis for identifying Twist1 target genes in human hepatocellular carcinoma (HCC). The blue diagram represents Twist1 co-expression genes from TCGA, and the green diagram represents Twist1 targeting genes by chromatin immunoprecipitation–sequencing (ChIP-seq). c TP expression was significantly upregulated in LIHC samples compared with the normal liver tissues. d Correlation analysis of Twist1 and TP expression in clinical HCC specimens. e Relative TP expression analysis in seven HCC cell lines. The ratio of densitometry value to the corresponding glyceraldehyde 3-phosphate dehydrogenase (GAPDH) value was used to indicate relative protein expression. f Western blot used to analyze TP protein levels influenced by Twist1 in PLC-PRF-5 and Hep3B cells. g Twist1 promoted TP gene transcription as detected by luciferase assay. h Diagram of the ChIP-seq binding peaks related to the TP promoter. Binding site analysis showed two individual conserved motifs within the promoter of TP. i Luciferase assay results of Twist1 to the truncated TP promoter regions demonstrated that Twist1 bound to the TP promoters of region 3/4. j Luciferase assay results of Twist1 to the mutated TP promoter regions demonstrated that Twist1 bound to motif 1/2 in the TP promoter. (mean ± SD; n = 3 in triplicate; **P < 0.01)