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. 2019 Jan 17;10(2):43. doi: 10.1038/s41419-018-1282-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Twist1 and TP promoted PLC-PRF-5 xenograft growth, whereas knocking down TP impaired the promotion effects of Twist1. b Number of tumors formed and lung metastasis in different groups. Twist1 and TP promoted lung metastasis of HCC, while knocking down of TP attenuated the promotion effect of Twist1. Images were taken at ×100 magnification. c Statistical analysis of VM formations in different groups. d Analysis of Twist1, TP, VE–Cad, vascular endothelial growth factor receptor 1 (VEGFR1), and VEGFR2 expression levels in xenograft tumors. Images were taken at ×400 magnification. e Statistical analysis of Twist1, TP, VE–Cad, VEGFR1, and VEGFR2 expression levels in different treated xenograft tumors (mean ± SD; *P < 0.05; **P < 0.01)