Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 20;9:4905. doi: 10.1038/s41598-019-41355-2

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Influence of CS, HA, SH, and HE on MMP2 enzyme activity. MMP2 enzyme activity was determined with rhMMP2 (100 ng/mL) and 50 µM fluorogenic peptide (MCA-Pro-Leu-Ala-Nva-Dpa-Ala-Arg-NH₂) as a substrate. Kinetic measurement was performed for 5 h without GAG (black curve) and in the presence of CS (A, green), HA (B, brown), SH (C, pink), and HE (D, teal) (200 µg/mL each; 20–1,000 µg/mL shown in Supplemental Fig. S5); blanks are given in light grey. (E) shows the endpoint values of MMP2 activity after 5 h; the horizontal line indicates the blank value. The results are presented as mean ± SEM. Significant differences of treatment vs. Ctrl were analyzed by one-way ANOVA/Bonferroni’s post-test and are indicated with ***(p < 0.001), n = 4.