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. 2019 Mar 18;9:4733. doi: 10.1038/s41598-019-41276-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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γ-Glu-Leu dissolved in MES buffer (pH 6.0) has no obvious effect on Ca²⁺ flux and the response to a second elicitor. (A,B) pMAQ2 seedlings were treated with MES (pH 6.0) or 500 µM γ-Glu-Leu (in MES buffer, pH 6.0) for approximately 15 min prior to the application of (A) 1-fold mycelium extract and (B) 1 µM flg22. [Ca²⁺]_cyt data of two independent experiments were pooled. Error bars represent standard error of the mean. ME = P. infestans mycelium extract.