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. 2019 Jan 15;10(2):35. doi: 10.1038/s41419-018-1270-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Diagram of the CREB1 3′-UTR luciferase reporter constructs containing wild-type or mutant miR-520b binding sites. B Relative luciferase activity in BGC823 cells cotransfected with wild-type or mutated reporter plasmids and miR-520b or controls. C PCR analysis of CREB1 mRNA expression in BGC823 and MKN45 cells transfected with miR-520b mimics and inhibitors, respectively. D Western blot analysis of CREB1 protein levels in the indicated GC cells. E Transwell assays of the migration and invasion abilities of BGC823 cells transfected with miR-520b, the CREB1 plasmid (CREB1) or the control plasmid vector (vector). F Western blot analysis of GATA6 and CREB1 expression in SGC7901 and MKN45 cells transduced with LV-GATA6 and LV-shGATA6 and an empty control vector, respectively. G Schematic structure of the CREB1 upstream promoter containing one GATA6-binding site. ChIP assays showing that GATA6 could not bind upstream of the CREB1 promoter region in SGC7901 cells. H Western blot analysis of GATA6 and CREB1 expression in BGC823 and SGC7901 cells infected with GATA6 or vector control and transfected with miR-520b inhibitor or control. I Negative correlation between miR-520b or GATA6 and CREB1 in GC tissues (n = 20). **p < 0.01, *p < 0.05. N.S. not significant (p > 0.05)