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. 2019 Mar 20;9:4925. doi: 10.1038/s41598-019-41346-3

Figure 3.

Figure 3

Inhibition of GSK3, which reduced CAP1 phosphorylation, also compromised cancer cell motility and invasion. (A) Treatment of PANC-1 cells with 6-BIO or LiCl reduced cell motility in the wound healing and Transwell migration assays. Treatment with 100 mM LiCl effectively reduced CAP1 phosphorylation in PANC-1 cells as well. Transwell migration assays with PANC-1 cells were conducted similarly as described in Fig. 2 where NT indicates cells without 6-BIO treatment. (B) Treatment with 100 mM LiCl also noticeably reduced CAP1 phosphorylation in AsPC-1 cells, as well as cell motility in the wound healing assays. (C) Treatment of PANC-1 cells with 5 μM 6-BIO significantly reduced cancer cell invasion in Matrigel invasion assays. Matrigel invasion assays, including data collection and analyses, were conducted similarly as described in Fig. 2D. (D) Knockdown of CAP1 in PANC-1 cells compromised the effect of 6-BIO in reducing cell motility in wound healing assays. Control and CAP1-knockdown stable PANC-1 clones were cultured for 16 hrs following the introduction of the wound, and the dashed lines indicate edges of the initial gap. Cell motility was quantified, analyzed using Student’s t-test, and plotted in the graph where error bars represent standard deviation. “*” indicates P < 0.05 and “**” indicates P < 0.01.