Fig. 5.

Gfi1b is required for the expression of Axin II- or TCF-dependent reporter alleles in HSCs and MKs in vivo (a–c) Axin2^+/LacZ Wnt-reporter mice were used. Littermates not carrying the reporter transgene (Axin2^+/+) were used to correct for endogenous β-galactosidase activity. a Mice were analysed by FACS at day 10 following the first injection of tamoxifen. b FDG (LacZ staining) histograms of indicated FACS gated cells. A representative result from three independent experiments is shown. c Quantification of the mean fluorescence intensity (MFI) measured in the FDG channel (LacZ) normalized to endogenous β-galactosidase activity. Paired t-test was used for the analysis. (n = 5 mice per group) (d, e) In vivo measurement of canonical Wnt signaling activity in Gfi1b WT/KO MKs and HSCs by FACS using TCF-H2B-GFP transgene reporter mice. A Mann–Whitney U non-parametric test was used for the analysis. Boxplot Centre line shows median and bounds of box and whiskers show interquartile ranges of GFP MFI, n = 5 GFI1b WT and five GFI1B KO mice