Fig. 7.

Canonical and non-canonical Wnt target genes are deregulated in Gfi1b KO MK in vitro. a Gfi1b WT/KO mice were taken to obtain lineage depleted BM cells, n = 2 GFI1b WT and GFI1B KO mice. Cells were taken into culture in the presence of TPO and SCF for 7 days and MK were separated using BSA gradient followed by RNA-seq analysis. b Volcano plot of expression fold change (KO/WT) over the P value of differences between WT and KO. Vertical dashed lines show the threshold of two-fold change in expression in KO samples compared to WT. The color code shows the co-occupation of promotor by indicated factors as seen in ChIP-seq data from K562 cells. c, d Gene set enrichment analysis (GSEA) of RNA-seq data from the sorted cells using published Wnt related gene sets and two new sets of Wnt target genes pooled from published data. e Unsupervised clustering of RNA-seq data using indicated gene sets