Table 1.
WGS-identified mutations in ceftazidime-evolved populations.
| Population | Gene | Localization | Type | Nucleotide change | Amino acid change | Frequency (%) | Detected day of emergence |
|---|---|---|---|---|---|---|---|
| A | smeH | 3061160 | SNV | C → A | P326Q | 98.7 | 5 |
| SMD_2719 | 3026930 | SNV | A → C | V232G | 51.6 | 20 | |
| phoQ | 315221 | SNV | T → A | I76N | 100 | 25 | |
| ftsI | 736289 | SNV | C → A | A592D | 100 | 25 | |
| SMD_0534 | 613290 | Ins | Ins of 1.037 bp | V398fs | — | 30 | |
| B | smeH | 3061160 | SNV | C → A | P326Q | 100 | 5 |
| smeH | 3062171 | SNV | A → G | Q663R | 100 | 20 | |
| SMD_0260 | 314100 | SNV | A → G | K88R | 70.1 | 25 | |
| C | smeH | 3061160 | SNV | C → A | P326Q | 100 | 5 |
| yrbC | 4744239 | SNV | C → T | Q126* | 32.3 | 30 | |
| yciM | 2035186 | Del | Del of 188 bp | A206fs | — | 30 | |
| phoQ | 315914 | SNV | C → T | S307L | 41.2 | 30 | |
| phoQ | 315965 | SNV | C → T | P324L | 37.5 | 30 | |
| D | smeH | 3061160 | SNV | C → A | P326Q | 100 | 5 |
| smeH | 3062171 | SNV | A → G | Q663R | 100 | 20 | |
| phoQ | 315236 | SNV | C → T | P81L | 99.2 | 20 | |
| yrbE | 4742678 | Del | ATCGCCGTCG → — | I49fs | 65.3 | 30 | |
| SMD_1278 | 14224481 | Ins | — →TGACTT | F91_G92insDF | 57.1 | 30 | |
| mrkC | 685444 | Del | GGCTTC → — | G187_F188del | 76.3 | 30 |
SNV: single nucleotide variant; Ins: insertion; Del: deletion; Frequency (%): percentage of reads that contain the variation within a heterogeneous population; * STOP codon.