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. 2019 Mar 20;9:4944. doi: 10.1038/s41598-019-41467-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of Aa on the concentration of H₂O₂. (A) Aa suspensions were mixed with H₂O₂ solutions. H₂O₂ concentrations were then tested after 30 minutes of reaction. (B) H₂O₂ concentrations of different bacterial mixtures were measured at 10-minute intervals. The enlarged section of the graph, illustrated as a bar chart, showed the H₂O₂ concentration after 30 minutes of reaction. CDM media without bacteria was used as a blank control. (C) Wells were divided into two parts by a transwell system. Ss, Aa or CDM medium were located at different places as shown in the figure. The H₂O₂ concentration in the bottom section was measured. *P ≤ 0.05, ***P ≤ 0.001, Student’s t-test. Means and standard deviations from triplicate experiments are shown.