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. 2019 Mar 20;9:4836. doi: 10.1038/s41598-019-41235-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Increased gene detection and improved exonic alignment rates for 10cRNA-seq compared to scRNA-seq. (A) Detection of murine Ensembl genes for mouse oligodendrocyte precursor cells (OPCs) and lung neuroendocrine-derived cells. (B) Detection of human Ensembl genes for MCF-10A cells and human breast cells. (C) Exonic alignment rate comparison for OPCs and lung neuroendocrine-derived cells. (D) Exonic alignment rate comparison for MCF-10A cells and human breast cells. Public scRNA-seq data were obtained from the indicated accession numbers: sc1 = GSE75330, sc2 = GSE60361, sc3a = GSE103354 (plate-based), sc3b = GSE103354 (droplet-based), sc4 = GSE66357, sc5 = GSE113197, sc6 = PRJNA396019. 10cRNA-seq data were aggregated from independent 10-cell samples (circles) and 10-cell equivalents from pool-and-split controls. Pool-and-split controls from the same day are indicated with non-circular markers corresponding to the shared day. Pairwise differences between 10-cell and single-cell methods were assessed by permutation test.