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. 2019 Mar 20;10(4):273. doi: 10.1038/s41419-019-1435-2

Fig. 4. CC-CAFs-induced macrophages promote migration of CRC cells and suppress NK cells function.

Fig. 4

a Transwell migration assay of SW48 cells induced by macrophages (magnification, ×100). The blue dot in picture indicated migrated tumor cells. b Proliferation of SW48 cells induced by macrophages was evaluated by CCK-8 assay. (n = 3). c Proliferation of SW48 cells induced by macrophages was evaluated by EdU incorporation experiment (n = 3), the proportion of EdU positive cells was quantified. CAF induced Mφ refers to macrophages induced by CAFs. d FACS analysis and quantification of SW48 cells killed by NK cells (n = 5). e FACS analysis of CD107a (+) cells in NK cells. f FACS analysis of CD27 (+) cells in NK cells. Error bars represent mean ± s.d.; **P < 0.01; ***P < 0.001; n.s. not significant; by unpaired two-sided Student’s t-test (af)